Dual Resonance Frequency Enhanced Electrostatic Microscopy (DREEM) is a variant of electrostatic force microscopy (EFM), which is an AFM based technique. A schematic illustration of the technique and sample images are shown below. Read more in the published article.

AFM is a powerful technique for studying protein-protein and protein-DNA interactions. However, a significant limitation to AFM and other high-resolution microscopy is the difficulty of identifying different proteins in multi-protein complexes. To resolve this, we are interested in combining the … Read more

Utilizing pre-steady state kinetics with various other biochemical techniques, the Erie lab worked toward a better understanding of the process and regulation of transcription elongation. Our past projects included:    

AFM provides us unique capability in revealing structural and functional relationship of DNA repair, including but not limited to, the specificity of the protein binding complex, the binding affinity, the DNA bending through bend angle analysis, the conformation analysis, and how … Read more

Transcription is the DNA directed synthesis of RNA, and is the first step in a series of events that leads to gene expression. In both prokaryotes and eukaryotes, an evolutionarily conserved class of enzyme known as RNA polymerase carries out … Read more

We have developed single-molecule FRET methods to study conformational dynamics of DNA-protein complexes as well as to analyze large multi-protein complexes related to DNA repair. Motivation Our group focuses primarily on structure-functions studies of the DNA mismatch repair pathway. Although our … Read more